Precast Gels Overview: Size Range & Resolution

 

Type of Gel Optimal Size Range 
bp
  Resolution       Special 
       Features

Spreadex®

 

  50-1000    1 - 4 bp     

       highest
       resolving 
       power

    EL 1200   250-800    4 bp  
    EL 800   200-500    4 bp  
    EL 600   150-350    4 bp  
    EL 500   100-300    4 bp  
    EL 400   65-250    1 bp*  

    EL 300

 

  50-200    1 bp*  
         
PCR CheckIT™   100-4000     10 bp

      fast runs
      and easy DNA
      recovery

         
GMA   100-450     1 bp       SSCP and 
      heteroduplex
      analysis
         
Clearose® RNA    500-10000 nt         RNA analysis
         
Poly(NAT)®   50-20000    

   2 -3%**

      fine 
      separations
      in broad size 
      ranges
    6 %   400-20000    
    9 %   180-800     
  12 %   60-300     
         
Short 
Fragment™
     <50     1 bp/nt       For high 
      resolution 
      analysis of   
      short 
      nucleic acids
         

 * on 8 cm running distance

** for fragments up to 2 kb

 
Precast Gel Ordering Information:

Please refer to pdfGels_Productlist.pdf171.23 KB


 

For each Application it´s own Gel

Type of GelsApplication
Spreadex®

 

  • Microsatellite Analysis
  • RFLP (Restriction Fragment Length Polymorphism)
  • Multiplex PCR
  • Competitive PCR
  • Allele specific PCR
  • RNA Analysis
  • GMO Analysis

 

 

   
PCR CheckIT™
  • PCR Checking
  • High Throughput PCR Analysis
  • DNA recovery
  • RFLP (Restriction Fragment Length Polymorphism)
   
GMA
  • SSCP (Single Strand Conformational Polymorphism)
  • Heteroduplex Analysis
   
Clearose® RNA
  • Easy for Northern Blotting
  • Qualitative RNA analysis
  • Analysis of RNA transcripts
  • mRNA and tRNA 500 nt - 10000 nt
   
Poly(NAT)®
  • RFLP (Restriction Fragment Length Polymorphism)
  • RAPD (Randomly Amplified Polymorphic DNA)
   
Short Fragment™
  • Oligonucleotide analysis
  • siRNA analysis

Different Formats are available:

AL's ready-to-use gels are available in 8 different formats. They allow the analysis of up to 400 samples per gel. 

AL-Diagnostic provides two main formats of gels:

Mini Gels

Wide Mini Gels
  
Wide Mini Gels have either 2 or 4 gels per presentation.

Mini 8/12 wells

mini 8 12 wells 1Gels available:
Spreadex®, PCR CheckIT™, Poly(NAT)®,
GMA, Clearose® RNA
 

Running distance:
87 mm  for 8 samples
57 mm for 12 samples

Wide Mini 2x13 and 4x13

wide mini 2x13 and 4x13 1

Gels available:
Spreadex®, PCR CheckIT™, Poly(NAT)™
2x13 only: Clearose® RNA, Short Fragment™, GMA

Running distance:
87 mm for 2x13
40 mm for 4x13

 

Wide Mini 2x25 and 4x25

wide mini 2x25 and 4x25 1

Gels available
Spreadex®, PCR CheckIT™, Poly(NAT)™, Clearose BG-XL, Short Fragment™
2x25 only: GMA

Running distance:
87 mm for 2x25
40 mm for 4x25

 

Wide Mini 2x104, 2x104L and 2x200

wide mini 2x104 2x104l and 2x200

Gels available:
PCR CheckIT™, Spreadex®

Running distance:
17 mm for 2x104L
  8 mm for 2x104 and 2x200